Analysis of granule-associated Starch branching enzyme IIb, involved in amylose extender mutation of maize
Abstract
Maize starch is an important industrial commodity. Starch synthase, phosphorylase, branching and debranching enzymes act in concert and results in a particular starch architecture. Starch branching enzymes play an important role in defining the overall starch structure. In maize endosperm, Starch branching enzyme IIb is predominant. The computational structure of Starch branching enzyme IIb has been determined. The putative substrate binding site and active site pore channel have been elucidated. As the enzyme is known to prefer amylopectin as substrate, the disachharide melibiose containing α- 1,6 glycosidic bond was docked on the modelled protein. The substrate binding site and active site channel has been discussed. Understanding of starch structure is essential for its modification and processing in forms, that are desirable for specific end-use applications. Generation of feedstock specific for a particular starch modification would result in environmentally-friendly starch production system by minimizing the requirement of chemicals for starch modifications. This is also necessary for allele mining to direct further breeding goals in this area.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
