Marker assisted recurrent selection for genetic male sterile population improvement in rice
In the present study, by deploying marker assisted recurrent selection (MARS) strategy, the improvement of IR58025B genetic male sterile maintainer population for drought and salinity tolerance was carried out to develop maintainers with drought and salinity tolerance. Individual crosses were performed among sterile plants of IR58025B maintainer population and donors of drought tolerance viz., Vandana (qDTY12.1) and FL478 (Saltol). F1 plants were confirmed for the presence of target genes/QTLs with the help of SSR markers and raised to produce F2 seeds. Selected F2 plants were harvested and seeds from each cross combination were mixed in equal proportions to generate population for the first random mating or recurrent selection cycle. The same process is continued up to 4th cycle and 200 selected plants were subjected to genotyping for drought and salinity with the help of reported SSR markers. About 48 plants were found positive for salinity tolerance, 17 plants were positive for drought and 10 plants were positive for both the traits. These lines were advanced by pedigree method of breeding for deriving maintainers with drought and salinity tolerance.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.