EMS induced mutagenicity in pollen mother cells of Sesbania Pea (Sesbania cannabina Poir.)

  • Girjesh Kumar and Nitisha Srivastava
Keywords: Sesbania cannabina, ethyl methane sulphonate (EMS), chromosomal aberrations, bridges.


Mutagenic effect of EMS on pollen mother cells of Sesbania cannabina has been investigated in the present cytogenetical
observation. The progenies were obtained from seeds treated with four different time durations (1, 3, 5, and 7 hours) of 0.5%
solution of ethyl methane sulphonate (EMS). Study revealed various types of chromosomal aberrations at different doses of
EMS. Increase in chromosomal aberrations was dose dependent manner. Pollen mother cells of Sesbania cannabina exhibited an
increased incidence of chromosomal bridges, unorientation, laggards and precocious movement etc. at each dose of treatment.
Anaphasic and telophasic bridges were major abnormalities and found to be highest at 3 hr dose of treatment. Anaphasic bridges
were more prominent than telophasic bridges and percentages of different types of bridges at anaphase and telophase were also
studied and scored.
Research Article