Assessment of genetic variation at soybean mosaic virus resistance loci in Indian soybean (Glycine max L. Merill) genotypes using SSR markers

  • Sudhir Kumar Gupta Nuclear Agriculture & Biotechnology Division Bhabha Atomic Research Centre Mumbai
  • Joy G Manjaya Nuclear Agriculture & Biotechnology Division Bhabha Atomic Research Centre Mumbai
Keywords: Soybean, genetic variability, soybean mosaic virus, SSR


Soybean mosaic virus (SMV) disease is one of the major diseases of soybean globally that hampers soybean productivity. In India, SMV disease is gaining importance and there is a need to breed the soybean cultivars resistant to SMV. In India, soybean cultivars resistant to SMV have been identified but source of resistance has not been characterized. Therefore, the current study was carried out to study the genetic variation at three SMV resistance loci in a set of SMV resistant and susceptible Indian soybean genotypes using the mapped SSR markers. A total of 13 SSR markers reported to be linked to three SMV resistance loci were screened on 23 soybean genotypes showing differential response to SMV disease. A total of 54 alleles were generated at 12 polymorphic SSR loci with an average of 4.5 alleles/locus. The number of alleles ranged from 2 to 7 and gene diversity for the polymorphic SSR markers ranged from 0.38 to 0.77 with an average of 0.60, indicating the presence of high genetic variation in the Indian soybean genotypes at SMV resistance loci. Cluster analysis grouped the 23 soybean genotypes into three major clusters and results of the Principal Coordinate Analysis also congruent well with the cluster analysis. In the clustering, most of the genotypes were grouped as per their pedigree suggesting that they may be carrying the same SMV resistance gene. This study showed that Indian soybean genotypes have sufficient genetic variability at the three SMV resistance loci and it would be helpful in the selection of suitable parents in breeding for SMV resistance.
Research Article