Molecular markers linked to fertility restorer gene in A2 cytoplasm male sterility of pigeon pea [Cajanus cajan]
In pigeonpea the cytoplasmic-genetic male sterility (CMS) system is considered to be a feasible approach for the development of hybrids. Identification of CMS lines and their putative restorers using molecular markers in the early stage of growth is an important and economical in long duration pigeonpea. Based on phenotyping, the 120 plants of F2 designated as fertile and sterile. Both visual and pollen staining phenotyping unequivocally exhibited 85 fertile: 35 sterile plants in F2 population. The segregation of F2 for fertility restoration of A2 cytoplasm indicated 3F:1S segregation indicating dominant monogenic control of fertility restoration. Bulk segregant analysis revealed that 10 of the 139 polymorphic decamer RAPD markers produced precisely distinct bands subjective to fertility restorer DNA bulk while, none of the SSR was found polymorphic between bulks. Two of them viz; OPC 7617 (GTC CCG ACG A) and OPK 3860 (CCA GCT TAG G) evinced clear and unambiguous bands for fertility restorations in F2 population at 0.5 and 1.0 cM, respectively. They could be exploited as a precise selection tool for seminal but stubborn character like restoration of fertility in male sterile lines that has germane significance in otherwise immensely important heterosis breeding in pigeonpea.
C. cajan, C. scarabaeoides, CGMS system, RAPD, SSR
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.