In-vitro screening and field validation of rice (Oryza sativa L.) genotypes for drought tolerance

  • S. Utharasu and C. R. Anandakumar
Keywords: Rice, polyethylene glycol, drought tolerance


In vitro screening using polyethylene glycol (PEG) was performed with matured seed-derived callus from six
elite rice varieties and eight advanced cultures to understand the response to drought stress. Murashige and
Skoog medium supplemented with 2 mg l-1
of 1-napthaleneacetic acid and 5, 10, 15 and 20 g l-1
of PEG 6000
were used to induce drought stress in callus. Significant differences were observed among the genotypes,
treatments and their interactions for callus morphology and fresh weight. The genotypes PMK3, RMD(R)1,
ARB6 , ARB7 and ARB8 were identified as the most tolerant to drought stress using principal component
analysis (PCA). A total of 48 crosses made from these genotypes showed significant yield increase over their
respective parents. The hybrids RMD(R)1/ARB7, PMK3/ARB7 and PMK3/ARB8 were identified as drought
tolerant using PCA analysis. From the study it is proved that in vitro screening method can be used to identify
drought tolerant rice genotypes.

Research Article