Genetic variability for karnal bunt (Neovossia indica) resistance in recombinant inbred lines of wheat
Parental genotypes H567.71 and WH542 and 107 recombinant inbred lines (RILs) were grown in field conditions during the rabi season 2010-11 and 2011-12. The numbers of infected grains were recorded and percentage of infected grains and coefficients of infection were worked out. A wide range of variation was observed among the RILs evaluated for Karnal bunt resistance. The distribution of RILs based on Karnal bunt disease was higher towards the resistant parent (H567.71). The population also characterized morphologically for various traits and analysis of variance for morphological characters showed significant variations among the RILs. Seventy SSRs markers were used for screening of the parental genotypes out of these 42 were found polymorphic. These 42 SSR markers were used to screen selected RILs and a total of 88 alleles were detected, number of alleles per locus ranged from 2-3 with an average of 2.09 alleles per locus. The NTSYS-pc UPGMA cluster analysis was used to determine similarity between parental genotypes and selected RILs based on the SSR scoring data. The dendrogram represented two major groups; 1st major group consist of H567.71 (resistant parental genotype) along with resistant RILs and the 2nd major group comprised of WH542 (susceptible parental genotype) and susceptible RILs at the similarity coefficient value of 0.39. Genetic relationship was also assessed by Principal component analysis (PCA) and similar results were obtained.
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