Validation of parents and estimation of molecular diversity through SSR Markers in maize (Zea mays L.)
Abstract
India is the third largest consumer of edible oils; an increasing human population increases the demand of oil at an alarming rate. Therefore, there is a need to search new sources of oil in India to fulfill this goal. Molecular markers have facilitated the easy identification of diverse parents for development of superior high oil lines for commercial use. The parental material in the present experiment comprised of ten diverse inbred lines viz., high oil (HIGH OIL, DMHOC-09, DMHOC-15), QPM or intermediate oil (HUZQPM-01, HUZQPM-03, HUZQPM-05, HUZQPM-06) and low oil (HUZM-53, HUZM-265, HUZM-478) content containing, selected on the basis of yield, yield traits along with oil content at the Agricultural Research Farm, B.H.U., during Kharif 2011 and were subjected to validate the presence of QTL’s linked to oil content and molecular diversity through SSR markers. A total number of 25 polymorphic SSR markers were used to evaluate molecular diversity among parental inbreds. The PIC of 25 SSR primers ranged from 0.42 to 0.50 with an average PIC of 0.46. The molecular validation studies revealed that SSR markers bnlg2086, phi96100, umc1422, bnlg1452, bnlg2244, bnlg1711, umc2319, umc1360, phi119 and phi050 were linked to QTLs for oil content. Therefore, these markers could be used in MAS for estimating high oil content in maize. The markers viz., nlg2086, phi96100, umc1422, bnlg1452, bnlg2244, bnlg1711, umc2319, umc1360, phi119 and phi050 were linked with QTL’s for oil content, were amplified corresponding oil content bands in most of the high and low oil inbreds. Therefore, these markers could be used in MAS for high oil content in maize. Total twenty five polymorphic micro-satellite markers were used to evaluate molecular diversity among parental inbreds. The PIC of the 25 SSR primers ranged from 0.42 to 0.50 with an average PIC of 0.46. The clustering of all the genotypes was according to their pedigree, oil content and other morphology.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
