Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
A total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that twelve mungbean and one urdbean germplasms were found to be highly susceptible (HS) and the rest from both mungbean and urdbean showed susceptible (S) to strain VA 239. One rice bean germplasm (TNAU Yellow) showed complete resistance. The accumulation of the viral genome in the host was confirmed by PCR amplification by using gene-specific primer. The screening results concluded that the resistant germplasm can be utilized as a donor in disease resistance breeding programme for generating MYMV resistant progenies in future.
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