Study on the impact of mutagenic treatment on pollen and spikelet fertility and its relationship in rice (Oryza sativa L.)
Abstract
The present study was conducted to study the impact of different mutagens on pollen and spikelet fertility. Pollen fertility and spikelet fertility per cent was found to be inversely proportional to increasing dose of the mutagen in all the treatments viz., gamma rays, EMS and its combination treatment. The range of per cent reduction in pollen fertility was observed from 16.67 per cent (100 Gy) to 26.62 per cent (300 Gy) in gamma rays treatment of the seeds. Reduction in pollen fertility due to EMS treatment and its combination treatment ranged from 13.57 per cent reduction (10 mM) to 24.16 per cent reduction (30 mM) and 20.62 per cent reduction (100 Gy + 30 mM) to 31.43 per cent reduction (300Gy + 30 mM) respectively. Spikelet fertility also showed similar relationship in all the treatments. The correlation coefficient between pollen and spikelet fertility was found to be positive and significant. The optimum dose of mutagen was also determined by using pollen and spikelet fertility per cent. The optimum dose was found to be 300 Gy, and 173 Gy + 30 mM for gamma rays and combination treatment respectively. Hence, this proves to be an easy method for determining optimum dose of mutagen.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
