Molecular and biochemical analysis of aroma in CSR10 x Taraori basmati derived Recombinant Inbred Lines
AbstractThe aroma or fragrance of Basmati rice is associated with the presence and content of chemical compound, 2-acetyl-1-pyrroline
and the trait is monogenic recessive. Several PCR-based co-dominant marker based on RG28 locus were developed, which can
differentiate between fragrant and non-fragrant rice cultivars. For molecular and biochemical analysis of aroma, a mapping
population comprising 208 recombinant inbred lines (RILs) derived from a diverse cross between CSR10 and Taraori Basmati
through SSD method was used. RILs are among the best mapping populations, which provide a novel material for linkage
mapping of genes/QTLs marker for various traits. Biochemical analysis of aroma was performed with the 1.7% KOH solution
and molecular analysis of aroma was carried out with microsatellite markers present on chromosome 8 (BAD2, BADEX7-5,
SCUSSR1) to determine the extent of association between trait, marker and chromosome 8. Among these markers, BAD2
amplified aroma specific alleles having 256 bp in 72 lines, BADEX7-5 with 95 bp in 74 lines and SCUSSR1 with 129 bp in 79
lines. Mentel test of significance detected by biochemical analysis of RILs (with 1.7 % KOH) and molecular marker study
revealed high degree (>90 %) of association of aroma with the above mentioned markers, respectively. Some of the F10 lines
amplified the heterozygous alleles for two sets of specific markers (BAD2 and SCUSSR-1) but did not show the presence of
aroma as analyzed by chemical test. Aromatic and non-aromatic lines were almost common in three markers indicating
association of markers with the trait and chromosome 8. The results revealed that these markers could be used for marker assisted
selection and RIL population for mapping of aroma QTLs/genes.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.