Morphological architecture of foliar stomata in M2 Carnation (Dianthus caryophyllus L.) genotypes using Scanning Electron Microscopy (SEM)

  • Rajib Roychowdhury, Parveen Sultana and Jagatpati Tah
Keywords: Dianthus caryophyllus, chemical mutagen, mutation breeding, stomata, Scanning Electron Microscopy.


Dianthus caryophyllus is an important floricultural crop in temperate climates and worldwide popular as cut-flowers for its
variegated petal’s colour. The development of this cultivar with more desirable floral characteristics and higher productivity are
also very much important. Their identifications as well as taxonomy had been studied in the literatures using different laboratory
methods. Both morphological and/or genetical characteristics were considered in the reported studies. However, to the best of our
knowledge, there does not exist any study involving an image analysis based approach. For this, we undertook the mutation
breeding programme with selected chemical mutagens, viz. Colchicine (COL), Ethyl Methane Sulphonate (EMS) and Maleic
Hydrazide (MH) with different concentrations. These mutagens were applied on the young leaves of M2 plants of Dianthus
cultivar. The results of the present study on peculiar morphological architectures of leaf stomata in Dianthus at different
concentrations of three potent chemical mutagens were analyzed on the basis of their Scanning Electron Microscopy (SEM)
images which is more informative than the classical approach. Number of stomata and its shape, aperture length and its
dimension, characteristics of guard cells in both dorsal and ventral surfaces of leaf also varied from treatment to treatment.
Research Note