Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE

  • Amit Kumar Dixit, Anshuman Singh, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade
Keywords: Maize, Genetic diversity, soluble seed protein, SDS-PAGE, Electrophoresis

Abstract

In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1.Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds.
Published
31-03-2015
How to Cite
Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade, A. K. D. A. S. (2015). Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE. Electronic Journal of Plant Breeding, 6(1), 264-268. Retrieved from http://ejplantbreeding.org/index.php/EJPB/article/view/662
Section
Research Note