Identification of Blast resistant rice (Oryza sativa L.) genotypes in indigenous and exotic germplasm and validation of Pi gene linked molecular markers
Abstract
Rice Blast is a serious disease caused by a fungal pathogen Magnaporthe grisea is one of the most devastating diseases of rice (Oryza sativa L.) in all rice growing areas of the world including India. The use of resistant varieties is considered as one of the most efficient ways of crop protection from the disease. Availability of resistant donors and validated molecular markers are essential to develop resistant cultivars against different races and to pyramid the resistant genes, In the present investigation 312 indigenous and 65 exotic germplasm lines were evaluated blast resistance at RARS, Jagtial. More percentage (83%) of exotic germplasm showed resistance to rice blast disease compared to indigenous germplasm (46%). Three genotypes (JGL23710, JGL23713 and JGL23714) in indigenous germplasm and two genotypes (IR09N500 and IR12M101) in exotic germplasm were immune to rice blast disease. These forms a donor genetic stock for development of highly resistant rice cultivars with high yields. Among five linked markers studied for Pi-1 gene one marker RM6094 was able to identify resistant genotypes at allelic level and for Pi-2 gene, RM527 was validated in four genotypes out of six genotypes used. This information will help rice breeders to improve the resistance to rice blast by marker assisted selection.
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