Diversity of proanthocyanidin content in soybean landraces
Keywords: Glycine max, antioxidants, genetic variation, proanthocyanidin, radical scavenging activities.
AbstractProanthocyanidins (PAs) are natural plant antioxidants, whose radical scavenging activities (RSA) degrade harmful free radicals. In the current study, PA contents were analyzed in 52 soybean [Glycine max (L) Merril] landraces having different seed coat colors. PA was extracted by HCl-Butanol method; RSA was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and quantified using spectrophotometer. Soybean cultivar KaiFengKuoZhuangqingDou had the highest PA content, 946 ABS.ml g-1, followed by Peking, 860 ABS.ml g-1. Both the cultivars had black seed coats. Chasengoku 81Go had the highest PA content (485 ABS.ml g-1) among the landraces with brown seed coat color. In landraces with green seed coats, PA content ranged from 1 to 6 ABS.ml g-1. In landraces with yellow seed coats PA content ranged from 0 to 10 ABS.ml g-1. The RSA was directly proportional to PA content with a high correlation coefficient (r2 = 0.9724), so PA content contributed highly to RSA. Reasons for these wide variations in PA content and RSA within the same colored landraces are discussed.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.