Analysis of seed storage protein diversity among twelve different cultivars of Pea (Pissum Sativum L.) using SDS-PAGE.
Abstract
The present investigation was carried out during 2012-13 with twelve Pea (Pisum sativum L.) cultivar for protein profiling through SDS-PAGE in The department of Genetics and Plant Breeding, CSA University of Agriculture and Technology, Kanpur (U.P). The Seed protein profiles of pea cultivars were studied by extracting the total proteins from five single seeds in each cultivar and performed SDS-Polyacrylamide gel electrophoresis. All the cultivars were clearly revealed remarkable polymorphism from their protein banding patterns. On the basis of banding patterns through SDS-PAGE, indicated that the number of bands found in cultivars ranged from 12 to 19 with Rm value 0.12 to 0.9. The results found that, the cultivar KPMR-400 had recorded highest number of bands (19) whereas, the minimum number of bands (12) observed in three cultivars viz., KPMR-921, KPMR-902 and KPMR-913. The total seed protein variation were also analyzed using Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) and resultant cluster analysis based on the data of protein profiling, classified twelve cultivars into six major groups. The finally study concluded that, the protein variability analysis clearly showed that there was sufficient genetic divergence among these cultivars of pea with respect to seed storage protein. Among all the cultivars, the KPMR-906 in cluster IV having wider genetic diversity and suggested to utilize in future crop improvement program.
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