SSR based genetic diversity in blast resistant and susceptible accessions of finger millet (Eleusine coracana.L)
Abstract
Finger millet (Eleusine coracana), serves as important millets in cereals and widely cultivated manly in arid and semiarid regions of the world. Presence of high amount of protein and calcium, it serves as a core staple food for rural populations in developing tropical countries where calcium deficiency and anemia are wide spread. In recent years, identification of polymorphisms at molecular level is being important for genetic diversity studies. Among several DNA based markers, SSR have become the marker of choice for this study. The present study was undertaken with the aim of investigating the applicability of available SSR markers for uncovered polymorphisms by using 25 primer pairs for varietal discrimination as well as for the genetic assessment in 32 finger millet accessions. Study revealed that all the SSR markers have showed polymorphism, out of which 17 were identified with a Polymorphic Information Content (PIC) value of above 0.3. The number of alleles per locus ranged from 2 to 5 with an average of 2.48 alleles per locus. Based on the similarity matrix values, the 32 genotypes were grouped into nine clusters at 0.44 Jaccard’s similarity coefficient. The similarity matrix values ranged from 0.20 to 0.72. The minimum similarity was observed between Indaf-5 and GE 1330 (0.20), and maximum similarity was observed between Indaf-9 and GE 71 (0.72). Based on the SSR markers, assessment of molecular diversity could serve as a sound basis in the identification of genetically distant accessions as well as in the duplicate scoring of the morphologically close accessions. Further, the identified polymorphic markers in the current study will serve as valuable source for further breeding programmes.
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