Spectrum and frequency of mutations induced by gamma rays and EMS in okra [Abelmoschus esculentus (L.) Moench]
Abstract
The seeds of okra variety P-8 were treated with different concentrations of gamma rays (65kR, 75kR and 85kR), EMS (1.2%, 1.4% and 1.6%) and their combinations to obtain the phenotypic response during M2 generation. Different mutation frequencies and width of mutation spectra were induced under the action of different concentrations of the applied mutagens. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses. Four different types of chlorophyll mutants, such as albino, xantha, viridis and chlorina were induced with effect of mutagens on plant population. The spectrum and frequency of chlorina mutants were highest followed by xantha, viridis and albino in all the treatments. The sole treatments of mutagens were found to be more efficient than combinations in inducing chlorophyll mutations. The highest frequency of chlorophyll mutations (0.044%) was reported in the 75kR+1.2% EMS. Based on the chlorophyll mutation frequency, it was found that EMS was most effective followed by gamma rays and combination of treatments.
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