In vitro mutagenesis creates distinct morphological variants in cassava (Manihot esculenta Crantz.): a characterisation study
Abstract
The present investigation was conducted to evaluate the variability created due to induction of in vitro mutagenesis in cassava. The calli and somatic embryos from the leaf explants of superior cassava genotypes Sree Jaya and CC1 established in Murashigue and Schoog (MS) media supplemented with 8 mg L-1 picloram were subjected to treatment with Ethyl Methyl Sulphonate (EMS), 0.6-1.2 per cent at 0.1 per cent interval. Forty-four plants survived out of sixty-eight regenerated plants kept for hardening under pad and fan greenhouse followed by rain shelter. Irrespective of the dosage field evaluation of these plants showed variations in morphological traits with respect to their respective controls at 3, 6 and 9 months after planting (MAP). These plants can be clonally propagated and the genetics of the observed variations in quantitative traits of these plants can be validated under different conditions.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
