Molecular diversity analysis of maize (Zea maysL.) inbreds using SSR markers
Abstract
Maize is the third most important cereals. Inbreds are the widely used parental material in the hybridization program. Many of these accessions have specific features, but only a few of them have been utilized in maize improvement programs.There is an important role of understanding the genetic diversity among and within inbred lines at the molecular level for maize improvement. The present investigation consists of 25 maize inbreds collected from the All India Co-ordinated Maize Improvement Project, Department of Genetics and Plant Breeding, IASc, BHU, Varanasi, were analysed for diversity using a total of 40 SSR markers during rabi 2015-16. Dendrogram was constructed based on UPGMA from the Jaccard’s similarity coefficient and the inbreds were subjected to cluster analysis. Among the 40 SSR markers used only 20 found informative (polymorphic) with total 70 alleles, provide a reference for determining the Simple Sequence Repeats (SSR) alleles number in genetic relationship analysis of maize inbred line and other crop germplasm. The PIC value ranged from 0.286 (umc226) to 0.966 (csu308) with an average PIC of 0.736. The first three PCs contributed 23.70, 7.63 and 6.73% respectively, with a cumulative variation of first 3PCs was 38.07%. The cluster analysis indicates inbred HUZM 252 and HUZM 265 are highly diverse. The present study has indicated the need for evaluating the component lines derived from each cluster. Cluster analysis indicates there is an enough diversity found between the inbreds tested and the information on diversity of inbred lines generated in this study would be much useful in developing heterotic hybrids.
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