Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
Abstract
Bacterial Leaf Blight (BB) is one of the devastating diseases in Southern India. An elite rice variety, CO43 which is a medium duration variety (135 days) with average yield (6.3t/ha) and does not show resistance to Bacterial Leaf Blight (BB). Scientists had discovered several BB resistant genes which are both dominant and recessive and is denoted as Xa and xa respectively. The current study is on the two genes Xa33 and Xa38 which are pyramided and is located on chromosome number 7 and 4 respectively. The rice genotypes FBR1-15 and PR114 were used as donors of Xa33, Xa38. The markers RMWR7.5 linked to Xa33 and Os04g53050-1 specific to Xa38 were validated in parents (CO43/ FBR1-15 and CO43/ PR114) and ICF3 populations to carry out Marker Assisted Selection (MAS). The segregating population of ICF3 were genotyped and the homozygous plants were subjected to artificial screening. The plants which showed resistance compared to CO43 are potential source of disease resistance which could be evaluated further and can be utilized for further breeding program.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
