Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense)
Proso millet and Little millet belong to group of small millets and family poaceae. Flower opening in these crops are chasmogamous type, in which pollination takes place prior to opening of flowers. Hence, self pollination predominates. So, to create variability, hybridization is prerequisite. Because of self pollination and non availability of male sterility, emasculation is necessary for crossing. There are many emasculation and crossing methods like contact method, hot water treatment, hand emasculation and USSR method which are used in these crops. But the disadvantage in these methods is damage to stigma this reduces the success rate of obtaining true F1’s. To overcome all these problems of earlier methods, in our present study, we proposed the modified crossing (SMUASB) method. In this method, cold water of 5-80C is sprayed on the panicle as a mechanical stimulator for opening of florets in male and female panicles. For emasculation, female panicle is gently washed in cold water. This will not affect stigma and its receptivity. All florets which are already fertilized and not opened immature florets are removed before pollination. Hence, success rate of obtaining true F1s is more and less land and resources are required for evaluation of F1’s. Using SMUASB method, we attempted crosses in Little millet and Proso millet and the success rate observed was 56% and 60% respectively.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.