Callus induction in Hydnocarpus pentandra (Buch. Ham.) Oken using shoot tips

  • Vignesha Bhat,
  • P. E. Rajasekharan, ICAR IIHR
  • G. Raviraja Shetty, Sadashiv Nadukeri and M. Ganapati

Abstract

Hydnocarpus pentandra (Buch. Ham.) Oken is a Vulnerable medicinal tree found in Western Ghats of India. It is highly exploited due to its medicinal properties and it is mainly used in leprosy treatment. An experiment was undertaken at Division of Plant Genetic Resources, ICAR-Indian Institute of Horticultural Sciences, Bengaluru during 2018-19. The experiment was laid down in Completely Randomized Design (CRD) with 16 treatments and three replications. Shoot tips from young seedlings were used as explant and treated in MS media with a different hormonal combination like Benzyl Amino Purine (BAP), Indole Acetic Acid (IAA), Kinetin and Thiaduzerol (TDZ). Among the treatments, highest shoot length (2.06 cm) and maximum number of shoots produced per explant (3.86) were found in the treatment combination of BAP (1.0 mg/l) and TDZ (0.5 mg/l) followed by the treatment combination of BAP (2.4 mg/l) and IAA (0.05 mg/l). Few treatments showed callus formation but no shoots were developed in these treatments.

Published
06-04-2020
How to Cite
Vignesha Bhat, P. E. Rajasekharan, G. Raviraja Shetty, Sadashiv Nadukeri and M. Ganapati
Callus induction in Hydnocarpus pentandra Buch. Ham. Oken using shoot tips. 2020. Electronic Journal of Plant Breeding, 11 01, 290-292. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/3221
Issue
Vol 11 No 01 (2020): EJPB
Section
Research Note

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