Standardization of vitrification treatments for safe cryo-conservation of banana (Musa acuminata Colla) cultivars using differential scanning calorimetry
Abstract
Cryo-conservation is the most promising approach and reliable method for long term conservation of banana and plantain genetic resources. Prior to the development of a safe cryo-conservation procedure for highly hydrated tissues of any vegetatively propagated crop species such as banana, suitable vitrification treatments involving loading solution in combination with Plant Vitrification Solution 2 (PVS2) solution have to be standardized. In the present study, Differential Scanning Calorimetry (DSC) technique was attempted to identify the best vitrification treatment using shoot apices as storage explants in two banana cultivars namely Grand Naine and Red banana. The results showed that DSC thermogram curves detected the formation of ice nucleation at a temperature of -18.67°C for Grand Naine and -19.16°C for Red banana in the control shoot apices. Among the sixteen vitrification treatment combinations tested, the treatments, LP6, LP7, LP10, LP11, LP14 and LP15 were judged as best treatments for cryo-conservation of both banana cultivars as revealed by the formation of glass transition events in the vitrified shoot apices. This information obtained through DSC experiments would further provide scope for designing safe cryo-conservation system for banana. This is the first report to demonstrate the application of DSC to optimize the vitrification treatments that would be adopted for developing cryo-conservation protocol for Musa germplasm.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
