Shortlisting putative candidate genes underlying qDTY1.1, a major effect drought tolerant QTL in rice (Oryza sativa L.)

  • Krishna Sai Karnatam, Deepa Jaganathan, Kambale Rohit Dilip, Manikanda Boopathi N and Raveendran Muthurajan TAMIL NADU AGRICULTURAL UNIVERSITY

Abstract

Drought is one of the major constraints affecting rice (Oryza sativa L.) productivity and production. Conventional breeding is leading to slow progress in developing drought tolerant varieties. In this context, mapping consistent major effect QTLs and unraveling molecular basis of those QTLs will pave way for deployment of those QTLs into breeding applications. Out of more than 2500 drought tolerance related QTLs, hardly 5 to 10 QTLs have been put into breeding applications due to larger QTL window, lack of tightly linked markers and lack of knowledge on candidate genes underlying QTLs. In this present study, in silico analysis was carried out to characterize a major effect QTL qDTY1.1 region on chromosome 1 for its gene content, drought responsiveness of genes in the QTL window and metabolic functions. Out of 352 genes residing in the qDTY1.1 window, 50% of the genes were found to be drought responsive. Bioinformatics search identified precise drought responsive expression pattern of 100 genes in the QTL window. Fifty seven genes were found to be up regulated and forty three genes were found to be down regulated. The qDTY1.1 region is found to contain 23 drought responsive transcription factors such as WRKY, bZIP, NAC, AP2 and C2H2. These drought responsive genes are confirmed for the possession of drought responsive cis elements like DRE, ABRE, MYB and MYC.

 

Published
30-09-2020
How to Cite
Krishna Sai Karnatam, Deepa Jaganathan, Kambale Rohit Dilip, Manikanda Boopathi N and Raveendran Muthurajan
Shortlisting putative candidate genes underlying qDTY1.1, a major effect drought tolerant QTL in rice Oryza sativa L.. 2020. Electronic Journal of Plant Breeding, 11 03, 916-924. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/3651
Issue
Vol 11 No 03 (2020): EJPB
Section
Research Article

It is certified that:

  • The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
  • The article has been seen by all the authors who are satisfied with its form and content.
  • The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
  • All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
  • The corresponding author takes full responsibility for this article.
  • The address of the organization where the research was conducted is given.
  • The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
  • Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
  • If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.