Resolving the acute gamma irradiation and ethyl methanesulphonate induced lethality for Jasminum sambac L. (Aiton) cv. Ramanathapuram Gundumalli
Crop improvement in Jasminum sambac through induced mutagenesis entails the insight on the sensitiveness of the individual plant materials of a species to different classes and nature of the mutagens. The current assessment was undertaken to analyse the mutagenic effect of acute gamma irradiation and EMS in Jasminum sambac cv. Ramanathapuram Gundumalli. Semi-hardwood cuttings of the plants were exposed to different dosage levels of gamma rays varying from 0 Gy (control) to 40 Gy and decapitated rooted cuttings were treated with EMS from 0 mM (control) to 90 mM. The M1V1 generation was evaluated by treating the cuttings with four different doses of gamma rays (15 Gy, 20 Gy, 25 Gy and 30 Gy) and three concentrations of EMS (45 mM, 60 mM, 75 mM) formulated using respective LD50 doses. The lethality dosage (LD50) value for the cultivar Ramanathapuram Gundumalli was determined to be 21.37 Gy and 64.57 mM for gamma irradiation and EMS, respectively applying the probit curve analysis. The maximal reduction in the survival of cuttings of 75% and 71% correspondingly in gamma and EMS treatments was discerned at higher doses in comparison to the lower doses. The plants studied in M1V1 generation for growth and flowering characters exhibited variable responses pertaining to differing doses or concentrations of the specific mutagenic means.
Keywords: Jasminum sambac, gamma irradiation, EMS, LD50 and semi-hardwood cuttings
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