Genetic Diversity Analysis of CIMMYT Bread Wheat (Triticum aestivum L.) Lines by SRAP Markers
AbstractGenetic diversity is one of the key factors for the improvement of many crop plants including wheat. Many wheat scientists
have studied genetic diversity in wheat germplasm using different molecular markers which have provided a powerful
approach to analyze genetic relationships among wheat germplasms. In this study, genetic diversity of CIMMYT
(International maize and wheat improvement center) bread wheat lines collected from Russia was evaluated using 30
sequence-related amplified polymorphism (SRAP) primer combinations. 686 DNA band was obtained from the 23 primer
combinations and approximately 90% of them were found to be polymorphic. Ratio of polymorphic loci, Shannon's
diversity index and gene diversity were found 82.61%, 0.39 and 0.26 respectively. The three main clusters were found by
using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis method and the average rate of
genetic similarity with 0.462. Two main clusters were shown in principal component analysis (PCA) which is consistent
with the result of UPGMA. It can be concluded that SRAP markers can be used for wheat genetic diversity studies and have
potential linkage mapping, molecular characterizations and marker assisted selection (MAS) breeding.
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