Identification of anaerobic germination tolerant landraces and validation of molecular marker in rice (Oryza sativa L.)
Poor germination and late seedling establishment are the main obstacles observed for wide spread adoption of direct-seeded rice cultivation in both rainfed and irrigated regions due to the waterlogged condition of the soil. The present experiment was carried out to identify rice landraces with anaerobic germination tolerance for the development of varieties suitable for direct seeded rice cultivation. A total of 53 genotypes of rice landraces were evaluated for anaerobic germination tolerance by following the screening methods of petri plate and tray method. The genotypes namely savudu samba, vellai kavuni, varappu kudaichan and korumbaali recorded the maximum germination percentage (> 90%) under anaerobic conditions and these genotypes also produced high dry matter production. The minimum germination percentage was recorded by malai nellu, surakuruvai and mappillai samba and the remaining landraces observed moderate germination percentage under anaerobic germination. Shoot length recorded for all the genotypes ranged from 0.70 to 6.23 cm under anaerobic germination. Dry matter production of genotypes ranged from 0.031 to 0.265 g/10 seedlings. Based on the screening, the best and least performed genotypes were selected for genotyping using already reported SSR markers. The marker produces the polymorphism between tolerant and susceptible genotypes, which can be exploited further for Marker Assisted Breeding programme.
Keywords : Rice, Landraces, Anaerobic, Germination, Validation, Molecular marker.
Identification of anaerobic germination tolerant landraces and validation of molecular marker in rice Oryza sativa L.. 2022. Electronic Journal of Plant Breeding, 13 3, 873-881. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/4373
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