Assessment of Quality Protein Maize (QPM) inbreds for genetic diversity using morphological characters and simple sequence repeats markers
An investigation was performed to characterize 25 indigenous Quality Protein Maize (QPM) inbred lines for several morphological traits to study the level of genetic diversity present in the inbreds by utilizing morphological characters and microsatellite markers. On the basis of morphological traits by adopting D2 statistics and Tocher's method of clustering all the studied inbred lines were clustered into seven clusters and by using molecular markers, based on Jaccard's similarity coefficient, these were clustered into five clusters. The inbred lines MTCA 1, MTCA 2, MTCA 8, MTCA 6, and MTCA 11, were grouped together, indicating that these inbred lines of maize were closely related to one another in terms of morphological and molecular characterization. Out of the total primers studied, five were observed with more than 0.5 PIC value. SSR primers utilized for the molecular characterization of inbred lines with the mean PIC value of 0.543, were quite informative. A total of 41 alleles were observed with an allele count ranging from 2-5 per locus. The 13 SSR primer pairs used for estimation yielded a total of four unique alleles, with a mean of 0.31 unique alleles per primer. The similarity coefficient estimates for pairs of individuals varied from 0.000 to 1.00. Among the pair-wise combinations of entries evaluated, the magnitude of the similarity coefficient between MTCA 11 and MTCA 10 (1.00) was the highest. However, the molecular data and the cluster diagram based on morphological data were at odds which may be due to the enormous genome size of a cross-pollinated crop like maize.
Keywords: QPM, SSR, maize, cluster, polymorphic, molecular
Assessment of Quality Protein Maize QPM inbreds for genetic diversity using morphological characters and simple sequence repeats markers. 2023. Electronic Journal of Plant Breeding, 14 3, 1276-1284. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/4869
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.