Sensitivity of seedling growth, fertility and yield contributing traits in rice (Oryza sativa L.) to electron beam and Ethyl Methane Sulphonate
Rice (Oryza sativa L.) plays a pivotal role in global food security, necessitating continuous efforts to enhance yield potential and grain quality. Mutation breeding, employing physical (electron beam) and chemical (ethyl methane sulphonate, EMS) mutagens, offers a promising avenue for creating genetic variability. This study assessed the sensitivity of rice variety ASD 16 and landrace Norungan to varying doses of electron beam (100 Gy to 500 Gy) and EMS (30 mM to 70 mM). The genotypes exhibited dose-dependent responses in germination, seedling survival, shoot and root length, seedling height, pollen fertility and spikelet fertility. The LD50 values for electron beam under in vivo and in vitro conditions were 314.24 Gy and 348.21 Gy respectively for ASD 16 and 445.07 Gy and 461.88 Gy for Norungan whereas for EMS, the LD50 values for ASD 16 were 49.17 mM and 55.37 mM; 53.53 mM and 65.12 mM for Norungan under in vivo and in vitro conditions based on probit analysis. ASD 16 showed a GR50 value of 309.02 Gy for the electron beam and 51.60 mM for EMS while Norungan exhibited a GR50 value of 538.79 Gy for electron beam and 98.71 mM for EMS. The variety, ASD 16 demonstrated higher sensitivity, proved by greater growth reduction percentages and lower lethal dose 50 (LD50) values compared to landrace Norungan. Electron beam treatments induce significant reductions in various growth traits, underlining the potential deleterious effects at higher doses. EMS, as a chemical mutagen, also impacted germination and growth characteristics. Both mutagens affected pollen and spikelet fertility with notable influence on yield contributing traits. This finding emphasizes the significance of precise dosage selection in mutation breeding to induce desirable traits and maintaining complete plant viability and productivity. Understanding genotype-specific responses is crucial for successful mutation breeding programmes. Overall, this study offers valuable insights into the application of electron beam and EMS mutagenesis in rice, contributing to the optimization of mutagenic protocols for crop improvement.
Keywords: : Rice, mutation, Electron beam, EMS, LD50, GR50,
Sensitivity of seedling growth, fertility and yield contributing traits in rice Oryza sativaandnbsp;L. to electron beam and Ethyl Methane Sulphonate. 2024. Electronic Journal of Plant Breeding, 14 4, 1505-1514. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/5001
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.