Genetic profiling of sugarcane genotypes using Inter Simple Sequence Repeat (ISSR) markers
AbstractStudy of genetic diversity is an important factor in sugarcane breeding programs to identify genetically diverse parents for
developing hybrid cultivars with improved cane and sugar yield. Genetic profiling studies of 17 sugarcane genotypes were
carried out by using 27 ISSR markers having di, tri and tetra nucleotide repeat motifs. Out of the 252 amplicons amplified by 27
ISSR primers, 212 were polymorphic (84.13%) with an average of 9.3 alleles per locus. The polymorphic information content
(PIC) value ranged from 0.19 (UBC 808, 843) to 0.36 (UBC876) primers with poly 'AG', 'CT' motifs and poly '(GATA) (GACA)'
motifs respectively with an average of 0.27. The primer UBC 825 had a highest resolving power value 9.8 among the 27 primers.
Genetic similarity indices by Jaccard’s similarity coefficient ranged from 0.26 to 0.72 indicating a high level of genetic diversity
and significant correlation found with biometrical data of sugarcane genotype. Cluster analysis by UPGMA method revealed
similarity coefficient of 0.49 which was mainly attributed to inter specific diversity. Thus, ISSR markers can be used as a
potential marker system for genetic profiling of sugarcane genotypes.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.