Analysis of genetic diversity in sesame (Sesamum indicum L.) germplasm lines based on agro-morphological traits and SSR markers
DOI: 10.37992/2024.1504.105
Abstract
An investigation was carried out with a set of fifty sesame genotypes to study the degree of genetic diversity based on morphological and marker based genotypic data. The genomic isolation was carried out using CTAB method and the molecular diversity was estimated with the help of fifty SSR markers using per cent polymorphism, PIC content and represented in dendogram. Morphological diversity was estimated by using D2 analysis. Quantitative traits, namely plant height (67.8-147.2 cm), number of capsules per plant (36-89), number of seeds per capsule (46-70), seed yield per plant (3.20-10.89 g), oil content (40.64-50.51 %), protein content (20.5-32.42 %), oleic acid content (31.25-42.85 %) and linoleic acid content (40.39-54.11 %) showed a significant amount of variation. Fifty genotypes were grouped into fourteen clusters based on data of 15 agro-morphological traits by following Tocher′s method. SSR markers showed 36% polymorphism with an average of 2.39 alleles per locus and 0.34 PIC value indicated a trend of moderate level of genetic diversity at molecular level in the collection of genotypes. In order to assess genetic diversity and to select various lines for breeding programs, the results highlighted the importance of utilizing both agro-morphological and molecular data.
Keywords: Sesame, genetic diversity, molecular markers, D2 analysis
Analysis of genetic diversity in sesame Sesamum indicum L. germplasm lines based on agro-morphological traits and SSR markers
. 2025. Electronic Journal of Plant Breeding, 15 4, 962-971. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/5194It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.