Comparative mutagenesis and effect of EMS, Gamma rays and Electron beam on biological parameters of Kalbhat and Black rice (Oryza sativa L.) non-basmati aromatic rice landraces

DOI:10.37992/2025.1602.027

  • Ranjit K. Londhe,
  • Shitalkumar P. Desai,
  • Akesh G. Jadhav,
  • Vinod J. Dhole,
  • Vishwas A. Bapat and,
  • Nikhil B. Gaikwad

Abstract

Kalbhat and Black rice are two  non-basmati scented rice landraces famous for their taste, aroma, and nutritional properties. Mutation was attempted by using ethyl methanesulfonate, gamma radiation, and electron beam in Kalbhat and Black rice. In M1 generation studies, the effect of mutagens on several biological variables was studied, like lethality, chromosomal abnormalities, pollen sterility, and plant survival. In M2 generation studies, different chlorophyll mutants like Albina, Xantha, Chlorina, and Viridis were identified. The frequency of chlorophyll mutants was relatively higher in Black rice (9.54) as compared to Kalbhat (7.72). Mutagenic effectiveness and efficiency with respect to seedlings height, lethality, and pollen sterility were studied, and the maximum effectiveness and efficiency were observed in EMS treatment in both Black rice and Kalbhat.The  lethality and pollen sterility was maximum in EMS treated  Kalbhat, while  mutagenic efficiency in terms of seedlings height and pollen sterility were maximum in the electron beam treated black rice. EMS was found to be more effective and efficient than gamma radiation and electron beam treatment in both the landraces.

Keywords: Black rice, Effectiveness, Kalbhat, landraces, Mutation frequency.

Published
04-07-2025
How to Cite
Ranjit K. Londhe, Shitalkumar P. Desai, Akesh G. Jadhav, Vinod J. Dhole, Vishwas A. Bapat and, Nikhil B. Gaikwad

Comparative mutagenesis and effect of EMS, Gamma rays and Electron beam on biological parameters of Kalbhat and Black rice Oryza sativa L. non-basmati aromatic rice landraces

. 2025. Electronic Journal of Plant Breeding, 16 2, 216-224. Retrieved from https://ejplantbreeding.org/index.php/EJPB/article/view/5328
Issue
Vol 16 No 2 (2025): EJPB
Section
Research Article

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