SSR, ISSR and RAPD markers based assessment of genetic diversity in aethiopicum and melongena species of genus Solanum
Abstract
The genetic diversity among 14 genotypes of Solanum melongena and Solanum aethiopicum was assessed using 40 polymorphic markers of SSR, ISSR and RAPD. Twelve RAPD markers with polymorphism percentage of 63.0 to 100.0 amplified 7.75 loci per marker with a total of 93 polymorphic bands. Sixteen polymorphic ISSR markers revealed 73 polymorphic loci with 4.56 polymorphic loci per marker and polymorphism percentage of 43.0 to 100.0 whereas 12 SSR markers amplified 43 polymorphic alleles with average polymorphic alleles per marker of 3.58. Forty markers of RAPD, ISSR and SSR detected a total of 57 unique/novel alleles with aethiopicum whereas 19 unique/novel alleles were generated in different genotypes of melongena. Each marker system individually as well as altogether exhibited least similarity coefficients of 0.24 to 0.29 between aethiopicum and melongena species of Solanum whereas higher order of similarity was observed within melongena group. Dendrograms generated using marker data of RAPD, ISSR, SSR and RAPD+ISSR+SSR separated aethiopicum and melongena in two divergent groups. We did not observe exactly similar pattern of grouping of different genotypes of melongena group in more homogenous cluster when different marker systems were compared. The Mantel test between the two Jaccard’s similarity matrices indicated high correspondence between RAPD and ISSR, RAPD and SSR, and ISSR and SSR based similarity matrices. Distribution of genotypes from different regions did not show clear clustering pattern when three marker systems were compared. Three dimensional plots of genotypes based on principal component analysis indicate close correspondence with UPGMA clustering approach.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
