Determination of optimum dose of chemical mutagen for large scale seed treatment of white seeded sesame (Sesamum indicum L.) varieties

V. Sandhiya
M. Kumar
C. Parameswari
C. Vanniarajan
N. Kumaravadivel
N. Sakthivel
Anand M. Badigannavar
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Abstract

In the present study two white seeded sesame varieties VRI 3 and SVPR 1 were treated with sevendifferent concentration viz., 0.2 %, 0.4 %, 0.6 %, 0.8 %, 1 %, 1.2 % and 1.4 % of chemical mutagen EthylMethane Sulphonate (EMS). Germination percentage and germination index were tested using two differentgermination methods. Seed germination percentage and germination index was decreased with increaseddoses of EMS in both the varieties. The higher concentration of EMS completely arrested the seedgermination. The optimum dose of the mutagen should be tested to maintain the require plant populatioin the M1 generation and increasing the effectiveness and efficiency of the mutagen. LD 50 value wasdetermined using probit analysis. For SVPR 1 and VRI 3, LD 50 value was found to be 0.36% and 0.44 %respectively. For fixing the lethal dose for the chemical mutagen, Pro tray method of germination test wasfound to be reliable than germination paper method.

Keywords White seeded sesame, EMS, Optimum dose, Germination percentage, Germination Index
Published 09/02/26