Analysis of simple sequence repeat (SSR) polymorphism between N22 and Uma rice varieties for marker assisted selection

P. Sindhumole
Swapnil Gorakh Waghmare
M. R. Shylaja
Deepu Mathew
Rose Mary Francies
P. S. Abida
S. Sajini
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Abstract

SSR polymorphism in two rice varieties viz., N22 (donor parent for heat and drought tolerance) and Uma (high yielding 
variety susceptible to heat and drought) was investigated to identify ready to use SSR markers which can be employed in 
selection in the marker assisted breeding programmes involving these varieties. For this, 197 simple sequence repeat (SSR) 
primers which were distributed on all the twelve chromosomes of rice were utilised. The results revealed distinct 
polymorphism among the tolerant and susceptible parents for 41 RM primers. Based on this study, the large range of 
similarity and dissimilarity values for N22 and Uma provides greater confidence for the assessment of simple sequence 
repeat (SSR) polymorphism. Diversity analysis can be done by using these polymorphic SSR markers. These polymorphic 
markers can also be used for the linkage analysis of various traits. Besides, these identified polymorphic SSR markers can be 
utilised for QTL mapping by screening the progenies of the cross Uma × N22.

Keywords Simple sequence repeats (SSR), parental polymorphism, N22, Uma, Marker assisted selection
Published 23/02/26